Title: The Chiptip: A Novel Tool for Automated Patch Clamp
VOLUME: 12 ISSUE: 1
Author(s):Andjelko Golubovic, Martina Knirsch, Uwe Czubayko, Alexander Hummer, Albrecht Lepple-Wienhues and Dirck Lassen
Affiliation:Flyion GmbH, Waldhauserstr. 64, D-72076 Tubingen, Germany.
Keywords:ligand gated channel, voltage gated channel, membrane protein, glass micropipettes, automated patch clamp, Ion channel
Abstract: To facilitate automated patch clamp measurements of ion channels in cells, the development of an all-glass Chiptip pipette is reported that may be combined with the previously described Flip-the-Tip technology. A single measurement requires less than 50 cells, and the addition of drugs for screening can be limited to very low volumes down to 1 μL. This apparatus is suitable for the study small cells, subcellular organelles and bacteria.
