Immobilised Metal Affinity Chromatography for the Analysis of Proteins and Peptides

Author(s): Gushinder Kaur-Atwal, Daniel J. Weston, Philip L.R. Bonner, Susan Crosland, Philip S. Green, Colin S. Creaser

Journal Name: Current Analytical Chemistry

Volume 4 , Issue 2 , 2008

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Immobilised metal affinity chromatography (IMAC) has become an established purification procedure for the selective isolation of proteins, peptides and post-translationally modified peptides, using a wide range of metal ions, including Cu(II), Fe(III) and Ga(III). Recently, the potential of interfacing capillary IMAC with mass spectrometry has been explored. This article reviews the development and use of IMAC in protein/peptide analysis, highlighting the benefits of capillary columns and their application to IMAC based enrichment.

Keywords: Immobilised metal affinity chromatography, Selective isolation, Protein/peptide analysis, Capillary column, Liquid chromatography-mass spectrometry

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Article Details

Year: 2008
Page: [127 - 135]
Pages: 9
DOI: 10.2174/157341108784587777
Price: $65

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PDF: 9