High Throughput Screening of Gene Functions in Mammalian Cells Using Reversely Transfected Cell Arrays: Review And Protocol

Author(s): Michael Sturzl*, Andreas Konrad, Gaby Sander, Effi Wies, Frank Neipel, Elisabeth Naschberger, Simone Reipschlager, Nathalie Gonin-Laurent, Raymund E. Horch, Ulrich Kneser, Werner Hohenberger, Holger Erfle, Mathias Thurau

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 11 , Issue 2 , 2008

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Reversely transfected cell microarrays (RTCM) have been introduced as a method for parallel high throughput analysis of gene functions in mammalian cells. Hundreds to thousands of different recombinant DNA or RNA molecules can be transfected into different cell clusters at the same time on a single glass slide with this method. This allows either the simultaneous overexpression or - by using the recently developed RNA interference (RNAi) techniques - knockdown of a huge number of target genes. A growing number of sophisticated detection systems have been established to determine quantitatively the effects of the transfected molecules on the cell phenotype. Several different cell types have been successfully used for this procedure. This review summarizes the presently available knowledge on this technique and provides a laboratory protocol.

Keywords: Reverse transfection, cell microarray, cell chip, apoptosis, proliferation, siRNA

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Article Details

Year: 2008
Page: [159 - 172]
Pages: 14
DOI: 10.2174/138620708783744499

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PDF: 33