Antibody-based Fluorescence Polarization Assay to Screen Combinatorial Libraries for Sweet Taste Compounds

Author(s): D. S. Linthicum, J. Patel, N. Cairns

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 4 , Issue 5 , 2001

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Abstract:

Technological advances in instrumentation, chemical synthesis methods, molecular biology and biochemistry have fueled the recent growth in high throughput screening. Assays are available in a vast range for formats, including fluorescence, luminescence, absorbance, and scintillation detection. Antibodies represent a powerful tool for novel compound discovery and their utility in this regard should not be underestimated. We have designed a fluorescence polarization immunoassay for the identification of novel sweeteners. The assay is based on monoclonal antibodies that bind superpotent sweet taste compounds and libraries of suitable test compounds can be rapidly screened using these antibodies as artificial taste receptors.

Keywords: antibody based fluorescence polarization assay, immunoassay, fluorescence polarization immunoassay fpia, fluorescence polarization immunoassay, high throughput screening hts

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Article Details

VOLUME: 4
ISSUE: 5
Year: 2001
Page: [431 - 438]
Pages: 8
DOI: 10.2174/1386207013331002
Price: $65

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