Biotechnology has been one of the scientific fields with the highest development during the last few decades which has
come from the integration of biological, physical and engineering sciences in order to achieve the technological application of
biological systems. Biotechnology is not an industry in itself, but an important technology that has a great impact on many
different industrial sectors. One of the advantages of the advanced biotechnology over classical biotechnology is that the mass
balance can be easily applied to biotechnological products and control mechanism can be easily studied. Recently, the
application of biotechnology is focused on enzymes’ production and its application. In the last few years, there have been very
rapid developments in genetic manipulation techniques which have introduced the possibility of 'tailoring' organisms in order to
optimize the production of established or novel enzymes of commercial importance.
Enzymes are a major resource utilized by the food, chemical, and allied industries to produce a wide range of
biotechnological products and have already been recognized as valuable catalysts for various organic transformations and
production of fine chemicals and pharmaceuticals. Most commercial enzymes are produced by microorganisms. The natural
ability of microbes to secrete high levels of enzymes has made them potentially attractive hosts for enzyme production.
Because of the wide application of this enzyme, intense research has been focused over the last half-century. However, research
studies have been carried out mostly from the point of view of selection of microorganism, production, purification and
application. On the other hand, little information is available on the effects of bioprocess design parameters for enzyme
production. In bioprocesses, besides the selection of the most potential producer, carbon and energy sources and their
concentrations are important as they are tools for bioprocess medium design. Additionally, bioreactor operation conditions such
as oxygen transfer rate, pH, temperature, inoculum percentage and incubation time show diverse effects on enzyme production
by influencing the metabolic pathways and changing metabolic fluxes [1-4].
Although considerable research works have been carried out in this area but the main emphasis has been given in this
thematic issue on strain improvement, production, purification and applications of the industrial enzyme. This thematic issue
will explain the various ways on strain improvement strategy along with the maintenance of microorganism for large-scale
production. In this thematic issue, classification and mode of action of various industrial enzymes including genetic regulation
and strategy for robust enzyme production have been discussed critically. In addition, process intensification by Computation
Fluid Dynamics (CFD) required for enzyme production is also covered in this thematic issue [5-7]. Furthermore, this thematic
issue also includes the recent patents and trends on strain improvement, production, purification and applications of industrial
enzyme and to update researchers, academicians, and industries on the recent developments/technologies in this domain.
