Large Antibody Display Libraries for Isolation of High-Affinity Antibodies

Author(s): Michael M. Ling

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 6 , Issue 5 , 2003

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Abstract:

Libraries of displayed antibodies have been increasingly used to select or screen antibodies. It is generally well recognized that large libraries provide high probability of finding a given antibody. In this review, the factors that limit the sizes of current in vivo and ribosome-based in vitro display libraries to be around 10 11 members are first discussed. An analysis resulting in a quantitative correlation between the library size and the antibody affinity is then presented. This analysis underscores the importance of large libraries in not only increasing the probability of finding a given antibody, but also enhancing the quality of a given antibody. Recent advances in preparing state-of-the-art large display libraries are then reviewed. Finally, potential improvements to current library technologies to generate libraries as large as 10 12 and their rationales are explored in length.

Keywords: antibody display libraries, high-affinity antibodies, ribosome-based

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Article Details

VOLUME: 6
ISSUE: 5
Year: 2003
Page: [421 - 432]
Pages: 12
DOI: 10.2174/138620703106298608
Price: $65

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