Design and Peptide-Based Validation of Phage Display Antibodies for Proteomic Biochips

Title: Design and Peptide-Based Validation of Phage Display Antibodies for Proteomic Biochips

Volume: 6 Issue: 1

Author(s): N. Stich, G. van Steen and T. Schalkhammer

Affiliation:

Keywords: epitope prediction, peptide synthesis, phage display antibody library, biochip, nanotechnology, proteomics

Abstract: To validate potential application of phage display-antibody arrays for high-throughput screening on a novel proteomics biochip, we examined the epitopes versus the full protein of glucose-6-phosphatedehydrogenase (G6PD) from yeast. In a predictive approach, we used the Hopp-Woods method and compared the results with antibodies directed against the entire enzyme. In total, 16 peptides of a length of 11 amino acids each fulfilling the desired criteria were identified and synthesized. Subsequently, antibodies against G6PD were raised using a phage display library. Selective interaction of the antibodies with certain peptides facilitated the identification of epitopes predicted by the hydropathic profile. The setup was adapted to a novel biochip system based on surface-enhanced absorption for direct CCD-camera based screening.

Cite this article as:

Stich N., Steen van G. and Schalkhammer T., Design and Peptide-Based Validation of Phage Display Antibodies for Proteomic Biochips, Combinatorial Chemistry & High Throughput Screening 2003; 6 (1) . https://dx.doi.org/10.2174/1386207033329841