Abstract
The genomics revolution has provided an overwhelming choice of potential drug targets for cancer therapy. Central to this revolution are approaches that identify target genes by changes in gene expression or through bioinformatic searching of DNA / protein databases for homologous sequences. An alternative to these approaches is functional cloning which selects gene products based on the ability of a given sequence to directly alter a cellular phenotype of interest. In an effort to discover new cancer therapeutic targets, bioinformatic searching and functional cloning were utilized to identify novel gene products implicated in various aspects of tumorigenesis. Using homology searches of DNA databases for novel death domain-encoding expressed sequence tags (ESTs), a new member of the TNF receptor family, DR6, was identified that regulated apoptosis in a variety of cell types and exhibited deregulated expression in tumor cells. Functional cloning of cDNAs from tumor cells with the capacity to induce morphological transformation, uncovered a unique oncogene, FGFR2-FRAG1, that resulted from a chromosomal rearrangemen t between a receptor tyrosine kinase, FGFR2, and a novel gene, FRAG1. This review focuses on these approaches as a means to identify potential targets for therapeutic intervention in cancer and the unique challenges each of these techniques face to link the identified gene products to a given biological effect or disease state.
Current Genomics
Title: Identifying and Validating Oncology Therapeutic Targets in the Post- Genomics Era
Volume: 4 Issue: 1
Author(s): Jian J. Lu and Matthew V. Lorenzi
Affiliation:
Abstract: The genomics revolution has provided an overwhelming choice of potential drug targets for cancer therapy. Central to this revolution are approaches that identify target genes by changes in gene expression or through bioinformatic searching of DNA / protein databases for homologous sequences. An alternative to these approaches is functional cloning which selects gene products based on the ability of a given sequence to directly alter a cellular phenotype of interest. In an effort to discover new cancer therapeutic targets, bioinformatic searching and functional cloning were utilized to identify novel gene products implicated in various aspects of tumorigenesis. Using homology searches of DNA databases for novel death domain-encoding expressed sequence tags (ESTs), a new member of the TNF receptor family, DR6, was identified that regulated apoptosis in a variety of cell types and exhibited deregulated expression in tumor cells. Functional cloning of cDNAs from tumor cells with the capacity to induce morphological transformation, uncovered a unique oncogene, FGFR2-FRAG1, that resulted from a chromosomal rearrangemen t between a receptor tyrosine kinase, FGFR2, and a novel gene, FRAG1. This review focuses on these approaches as a means to identify potential targets for therapeutic intervention in cancer and the unique challenges each of these techniques face to link the identified gene products to a given biological effect or disease state.
Export Options
About this article
Cite this article as:
Lu J. Jian and Lorenzi V. Matthew, Identifying and Validating Oncology Therapeutic Targets in the Post- Genomics Era, Current Genomics 2003; 4 (1) . https://dx.doi.org/10.2174/1389202033350119
DOI https://dx.doi.org/10.2174/1389202033350119 |
Print ISSN 1389-2029 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5488 |
Call for Papers in Thematic Issues
Advanced Computational Algorithms and Artificial Intelligence in Clinical Pharmacogenomics
In the era of personalized medicine, understanding the relationship between genetics and drug response is crucial. This issue delves into innovative methodologies, leveraging deep computational analysis and artificial intelligence, to enhance the field of Clinical Pharmacogenomics. The interdisciplinary approach harnesses the power of advanced high-throughput genotyping technologies, sophisticated computational analysis, ...read more
Applications of Single-cell Sequencing Technology in Reproductive Medicine
Single cell sequencing (SCS) technology utilizes individual cells' genetic material to sequence their genome, transcriptome, and epigenetics at the molecular level. It offers insights into cell heterogeneity and enables the study of limited biological materials. Since its recognition as a valuable technique in 2011, single cell sequencing has yielded numerous ...read more
Big Data in Cancer Research
Cancer is a significant threat to human life and health, remaining a highly aggressive killer. It is a leading cause of death worldwide and represents a crucial medical issue for humanity. However, in the past decade, the effectiveness of new synthetic anticancer agents has not matched the current clinical speculation. ...read more
Current Genomics in Cardiovascular Research
Cardiovascular diseases are the main cause of death in the world, in recent years we have had important advances in the interaction between cardiovascular disease and genomics. In this Research Topic, we intend for researchers to present their results with a focus on basic, translational and clinical investigations associated with ...read more
Related Journals
- Author Guidelines
- Graphical Abstracts
- Fabricating and Stating False Information
- Research Misconduct
- Post Publication Discussions and Corrections
- Publishing Ethics and Rectitude
- Increase Visibility of Your Article
- Archiving Policies
- Peer Review Workflow
- Order Your Article Before Print
- Promote Your Article
- Manuscript Transfer Facility
- Editorial Policies
- Allegations from Whistleblowers
- Announcements
Related Articles
-
Molecular Biology of Nucleoside Transporters and their Distributions and Functions in the Brain
Current Topics in Medicinal Chemistry Phenolics and Terpenoids; the Promising New Search for Anthelmintics: A Critical Review
Mini-Reviews in Medicinal Chemistry Strategies for the Biological Evaluation of Gold Anticancer Agents
Anti-Cancer Agents in Medicinal Chemistry Tetraplex Binding Molecules as Anti-Cancer Agents
Recent Patents on Anti-Cancer Drug Discovery The Use of the Zebrafish Model to Aid in Drug Discovery and Target Validation
Current Topics in Medicinal Chemistry Recent Updates in Imperative Natural Compounds for Healthy Brain and Nerve Function: A Systematic Review of Implications for Multiple Sclerosis
Current Drug Targets Endoscopic Microscopy Using Optical Coherence Tomography
Current Medical Imaging Lymphatic Targeting of Nanosystems for Anticancer Drug Therapy
Current Pharmaceutical Design Gadd45 Proteins as Critical Signal Transducers Linking NF-κB to MAPK Cascades
Current Cancer Drug Targets Radiopharmaceuticals Labelled with Copper Radionuclides: Clinical Results in Human Beings
Current Radiopharmaceuticals Potential Role of Rho Kinase Inhibitors in Combating Diabetes-Related Complications Including Diabetic Neuropathy-A Review
Current Diabetes Reviews Expression Profiles and Bioinformatics Analysis of Full-length circRNA Isoforms in Gliomas
Current Bioinformatics Reposition of the Fungicide Ciclopirox for Cancer Treatment
Recent Patents on Anti-Cancer Drug Discovery Recent Development in Identification of Potential Novel Therapeutic Targets Against Trypanosomatids
Current Topics in Medicinal Chemistry Mitochondrial Superoxide Dismutase: A Promising Target for New Anticancer Therapies
Current Medicinal Chemistry Molecular Evidence of Cryptotanshinone for Treatment and Prevention of Human Cancer
Anti-Cancer Agents in Medicinal Chemistry A Review on Natural Sources Derived Protein Nanoparticles as Anticancer Agents
Current Topics in Medicinal Chemistry Hemolytic Uremic Syndrome in Children
Current Pediatric Reviews Molecular Biomarkers for Lung Adenocarcinoma: A Short Review
Current Cancer Therapy Reviews New Insight Into A1 Adenosine Receptors in Diabetes Treatment
Current Pharmaceutical Design