Fluorescence Polarization Assays in Signal Transduction Discovery

Author(s): J. Richard Sportsman, Janet Daijo, Elizabeth A. Gaudet

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 6 , Issue 3 , 2003


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Abstract:

Fluorescence polarization (FP) has become widely employed for high throughput screening used in pharmaceutical drug discovery. Assays of important signal transduction targets are now adapted to FP. In this review we examine assays for cyclic adenosine monophosphate, phosphodiesterases, and protein kinases and phosphatases using FP competitive immunoassays and a direct enzymatic method called IMAP.

Keywords: Fluorescence Polarization, adenosine monophosphate, phosphodiesterases

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Article Details

VOLUME: 6
ISSUE: 3
Year: 2003
Page: [195 - 200]
Pages: 6
DOI: 10.2174/138620703106298374
Price: $65

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