(Review Article) Screening for Disease-Markers and Investigating Drug Effects by Proteome Profiling: Can it Meet Expectations?

Author(s): Christopher Gerner

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 7 , Issue 1 , 2004

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Abstract:

Drugs exert their functions mainly by affecting proteins. Therefore, it seems straightforward to focus on proteins in order to investigate drug effects. Unfortunately, proteins are of very high complexity, rendering it much more difficult to screen for protein alterations as compared to gene regulation. However, the efficiencyand applicability of proteome analysis has been dramatically increased recently. We are on the way to be ableto comprehensively assess disease-related proteome alterations, which may become an essential source ofinformation for knowledge-based drug design. This review will provide an overview of current techniques inproteome analysis, focusing on screening technologies for biomedical research. An outlook at the futurepotential of proteomics supported by modern bioinformatics will highlight why proteomics is worth the effort.

Keywords: 2d-page, autoradiography, fluorography, mass spectrometry, mudpit, quantitative, proteomics

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Article Details

VOLUME: 7
ISSUE: 1
Year: 2004
Page: [1 - 9]
Pages: 9
DOI: 10.2174/138620704772884779

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