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Current Pharmaceutical Biotechnology

Editor-in-Chief

ISSN (Print): 1389-2010
ISSN (Online): 1873-4316

Optimal Enzymes for Single-Molecule Sequencing

Author(s): Susanne Brakmann

Volume 5, Issue 1, 2004

Page: [119 - 126] Pages: 8

DOI: 10.2174/1389201043489611

Price: $65

Abstract

Much research effort has been made to realize a single-molecule sequencing. Central to this project are two enzymatic tasks that challenged several biochemists during the last years. They searched for polymerases for copying a DNA target into completely fluorophore-labeled DNA as well as for handling this new DNA derivative. Furthermore, they studied exonucleases for the sequential hydrolysis of completely dye-labeled DNA and explored optimal conditions for future application on the single-molecule level. In this article, the recent advances will be summarized from the biochemists point of view.

Keywords: dna sequencing, single-molecule detection, dna polymerase, exonuclease, fluorescence


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