Single-Molecule Spectroscopy for Nucleic Acid Analysis: A New Approach for Disease Detection and Genomic Analysis

Author(s): Peter M. Goodwin, Rhiannon L. Nolan, Hong Cai

Journal Name: Current Pharmaceutical Biotechnology

Volume 5 , Issue 3 , 2004

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Recently developed single-molecule spectroscopy (SMS) permits the analysis of fluorescent mixtures one molecule at a time. SMS methods provide the means to make rapid measurements on small, complex samples without the need for separations and target amplification enabling a new class of ultrasensitive nucleic acid assays. Here we give a brief overview of the current state of the art of SMS nucleic acid analysis and discuss ongoing work in our laboratory on two-color single-molecule fluorescence detection of specific nucleic acid sequences. In the future, two-color SMS nucleic acid assays will be used for a variety of applications including: gene expression analysis, disease detection and genomics.

Keywords: single-molecule, fluorescence, two-color, haplotyping, gene expression

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Article Details

Year: 2004
Page: [271 - 278]
Pages: 8
DOI: 10.2174/1389201043376814

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PDF: 15