Determination of the Antiretroviral Drug Nevirapine in Diluted Alkaline Electrolyte by Adsorptive Stripping Voltammetry at the Mercury Film Electrode

Author(s): Arnaldo Aguiar Castro, Ricardo Queiroz Aucelio, Nicolas Adrian Rey, Eliane Monsores Miguel, Percio Augusto Mardini Farias

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 14 , Issue 1 , 2011

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Abstract:

This paper describes a stripping method for the determination of nevirapine at the submicromolar concentration levels The method is based on controlled adsorptive accumulation of nevirapine at thin-film mercury electrode, followed by a linear cyclic scan voltammetry measurement of the surface species. Optimal experimental conditions include a 2.0 × 10-3 mol L-1 NaOH solution (supporting electrolyte), an accumulation potential of -0.20 V, and a scan rate of 100 mV s-1. The response of nevirapine is linear over the concentration range 0.01 – 0.14 ppm. For an accumulation time of 6 minutes, the detection limit was found to be 0.87 ppb (3.0 × 10- 9 mol L-1). More convenient methods to measure the nevirapine in presence of the efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine and metals ions were also investigated. The utility of this method is demonstrated by the presence of nevirapine together with ATP or DNA.

Keywords: Nevirapine determination, metals ions, efavirenz, acyclovir, didanosine, indinavir, nelfinavir, saquinavir, lamivudine, zidovudine, ATP, DNA, thin-film mercury electrode, linear cyclic scan stripping voltammetry, NNRTI, Houston Ametek-DMP-40 series digital plotter, Milli-Q, negative-going potential scan, voltammogram, Co-nevirapine complex, HIV

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Article Details

VOLUME: 14
ISSUE: 1
Year: 2011
Page: [22 - 27]
Pages: 6
DOI: 10.2174/1386207311107010022

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