Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques

Title: Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques

Volume: 5 Issue: 2

Author(s): Antonie J.W.G. Visser, Beno H. Kunst, Hans Keller and Arjen Schots

Affiliation:

Keywords: biolibrary, phage display, selection, sorting

Abstract: The selection of specific binding molecules like peptides and proteins from biolibraries using, for instance, phage display methods can be quite time-consuming. It is therefore desirable to develop a strategy that is much faster in selection and sorting of potential binders out of a biolibrary. In this contribution we separately discuss the current achievements in generation of biolibraries, single-molecule detection techniques and microfluidic devices. A highthroughput microfluidic platform is then proposed that combines the propulsion of liquid containing fluorescent components of the biolibrary through microchannels, single-molecule fluorescence photon burst detection and real-time sorting of positive hits.

Cite this article as:

Visser J.W.G. Antonie, Kunst H. Beno, Keller Hans and Schots Arjen, Towards Sorting of Biolibraries Using Single-Molecule Fluorescence Detection Techniques, Current Pharmaceutical Biotechnology 2004; 5 (2) . https://dx.doi.org/10.2174/1389201043376977