Art and Artefacts of Fluorescence Correlation Spectroscopy

Author(s): Jorg Enderlein, Ingo Gregor, Digambara Patra, Jorg Fitter

Journal Name: Current Pharmaceutical Biotechnology

Volume 5 , Issue 2 , 2004

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Fluorescence correlation spectroscopy (FCS) is an important technique for studying low concentrations of analyte molecules in solution. The core molecular characteristic that can be addressed by FCS is the translational diffusion coefficient of the analyte molecules, which can be used for i.e. studying molecular binding and reactions, or conformational changes of macromolecules. The present paper discusses several possible optical and photophysical effects that can influence the outcome of a FCS measurement and thus can bias the value of the derived diffusion coefficient.

Keywords: fluorescence correlation spectroscopy, diffusion coefficients, confocal detection

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Article Details

Year: 2004
Page: [155 - 161]
Pages: 7
DOI: 10.2174/1389201043377020

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PDF: 71