Phage Display and PEGylation of Therapeutic Proteins

Title: Phage Display and PEGylation of Therapeutic Proteins

Volume: 8 Issue: 2

Author(s): Yohei Mukai, Yasuo Yoshioka and Yasuo Tsutsumi

Affiliation:

Keywords: conjugation, pegylation, polyethylene glycol, polyvinylpyrrolidone, tumor necrosis factor-alpha, protein therapy, phage library, high throughput screening

Abstract: With the success of the human genome project, the focus of life science research has shifted to the functional and structural analyses of proteins, such as disease proteomics and structural genomics. These novel approaches to the analyses of proteins, including newly identified ones, are expected to help in the identification and development of protein therapies for various diseases. Thus, disease proteomic-based drug discovery has a very high profile. Nevertheless, the use of bioactive proteins in the clinical setting is not straightforward because, in vivo, these proteins have a low stability and a pleiotropic action. To promote disease proteomic-based drug discovery and development, we have attempted to establish a system for creating functional mutant proteins (muteins) with the desired properties, and also to develop a site-specific polymerconjugation system for further improving their therapeutic potency. These innovative protein-drug systems are discussed in this review.

Cite this article as:

Mukai Yohei, Yoshioka Yasuo and Tsutsumi Yasuo, Phage Display and PEGylation of Therapeutic Proteins, Combinatorial Chemistry & High Throughput Screening 2005; 8 (2) . https://dx.doi.org/10.2174/1386207053258578