Considerations on Antibody-Phage Display Methodology

Author(s): Udo Conrad, Jurgen Scheller

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 8 , Issue 2 , 2005

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For almost 15 years phage display is being used for the selection of specific antigen-binders from artificial libraries of single chain antibodies. Filamentous phages have been developed in a way to express foreign proteins on the surface and at the same time carrying the genetic information of the surface expressed molecule within the phage capsid. This property guarantees the coupling of phenotype and genotype during phage amplification and affinity selection. The possibility to generate large antibody libraries and the simplified antibody-backbone of a single chain antibody has made antibody-phage display to a powerful tool for the development of new therapeutics against various human diseases. In this review we discuss the general principles and latest developments and applications in antibody phage display technology.

Keywords: scfv, m13, phage display, affinity selection, library, antigen

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Article Details

Year: 2005
Page: [117 - 126]
Pages: 10
DOI: 10.2174/1386207053258532
Price: $65

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PDF: 10