High Performance Gene Delivery Polymeric Vector: Nano-Structured Cationic Star Polymers (Star Vectors)

Author(s): Yasuhide Nakayama, Takeshi Masuda, Makoto Nagaishi, Michiko Hayashi, Moto Ohira, Mariko Harada-Shiba

Journal Name: Current Drug Delivery

Volume 2 , Issue 1 , 2005


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Abstract:

Nano-structured hyperbranched cationic star polymers, called star vectors, were molecularly designed for a novel gene delivery non-viral vector. The linear and 3, 4 or 6 branched water-soluble cationic polymers, which had same molecular weight of ca. 18,000, were synthesized by iniferter (initiator-transfer agent-terminator)-based photo-livingradical polymerization of 3-(N,N-dimethylamino)propyl acrylamide, initiated from respective multi-dithiocarbamatederivatized benzenes as an iniferter. All polymers produced polyion complexes polyplexes by mixing with pDNA (pGL3-control plasmid), in which the particle size was ca. 250 nm in diameter [the charge ratio < 2 / 1 (vevtor / pDNA)] and ca. 150 nm (the charge ratio > 2.5 / 1), and the ζ-potential was ca. +10 mV (the charge ratio > 1 / 1). When COS-1 cells were incubated with the polyplexes 12h after preparation under the charge ratio of 5 / 1, higher gene expression was obtained as an increase in branching, with a little cytotoxicity. The relative gene expression to the linear polymer was about 2, 5, and 10 times in 3-, 4-, and 6-branched polymers, respectively. The precise change in branching of polymers enabled the control of the gene transfer activity.

Keywords: non-viral vector, star polymer, polyplex, branched polymer, gene transfection, molecular design

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Article Details

VOLUME: 2
ISSUE: 1
Year: 2005
Published on: 01 March, 2012
Page: [53 - 57]
Pages: 5
DOI: 10.2174/1567201052772825

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