Abstract
Narrowly size-distributed functionalized magnetic emulsions with diameters between 200 and 300 nm bearing reactive amine or carboxylic groups were prepared respectively via single adsorption or layer-by-layer adsorption process. The colloidal stability of the functionalized magnetic emulsion was related to the polymer adsorbed amount and to the adsorption methodology. The single adsorption of poly(ethyleneimine) onto negatively charged magnetic emulsion led to amino-containing magnetic nanodroplets for nucleic acid extraction, concentration and amplification. The enzymatic amplification of adsorbed nucleic acid molecules (i.e. RNA) was found to be related to both initial nucleic acid concentration and the used magnetic particles in the amplification step. The undesirable inhibition phenomena observed in the enzymatic amplification (i.e. RT-PCR) process was eliminated by the addition of appropriate negatively charged polyelectrolyte before the amplification step. The encapsulation of magnetic emulsion via layer-by-layer polyelectrolytes adsorption process was used to elaborate functionalized core-shell magnetic colloids. The characterized final magnetic dispersions were evaluated in specific nucleic acids capture and detection, and in proteins immobilization process.
Keywords: biomolecules, polystyrene magnetic particles, encapsulation, single polyelectrolyte adsorption, polycation, rt-pcr, hiv, amplification, streptavidin, enzyme-linked oligo-sorbent assay (elosa)
Call for Papers in Thematic Issues
Advances of Heterocyclic Chemistry with Pesticide Activity
Global food safety and security will continue to be a global concern for the next 50 years and beyond. Plant diseases have had a significant impact on food safety and security throughout the entire food chain, from primary production to consumption. While conventional chemical pesticides have been traditionally used for ...read more
Calculation design of covalent/metal organic framework based catalysts
This research area combines theoretical computation and screening with machine learning for the design of covalent/metal organic framework-based catalysts, bridging the disciplines of organic chemistry, physical chemistry, computational chemistry, materials science, and machine learning. It covers several critical aspects: designing and synthesizing organic catalysts for improved performance, applying computational methods ...read more
Carbohydrates conversion in biofuels and bioproducts
Biomass pretreatment, hydrolysis, and saccharification of carbohydrates, and sugars bioconversion in biofuels and bioproducts within a biorefinery framework. Carbohydrates derived from woody biomass, agricultural wastes, algae, sewage sludge, or any other lignocellulosic feedstock are included in this issue. Simulation, techno-economic analysis, and life cycle analysis of a biorefinery process are ...read more
Catalytic C-H bond activation as a tool for functionalization of heterocycles
The major topic is the functionalization of heterocycles through catalyzed C-H bond activation. The strategies based on C-H activation not only provide straightforward formation of C-C or C-X bonds but, more importantly, allow for the avoidance of pre-functionalization of one or two of the cross-coupling partners. The beneficial impact of ...read more
Related Books
Advances in Organic Synthesis
The Synthetic Methods, Structures, and Properties of the Ca-C σ Bond Organocalcium Containing Compounds
Advances in Organic Synthesis
Chemistry of Bipyrazoles: Synthesis and Applications
Advances in Organic Synthesis
Advanced Nanocatalysis for Organic Synthesis and Electroanalysis
Advances in Organic Synthesis
Advances in Organic Synthesis
4