Analytical and Biological Evaluation of High Throughput Screen Actives Using Evaporative Light Scattering, Chemiluminescent Nitrogen Detection, and Accurate Mass LC-MS-MS

Author(s): D.A. Peake, D.C. Duckworth, T.J. Perun, W.L. Scott, P. Kulanthaivel, M.A. Strege

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 8 , Issue 6 , 2005

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In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or “biofractionation” technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.

Keywords: Bioassay-guided fractionation, biofractionation evaporative light scattering detection (ELSD), evaporative light scattering detection (ELSD), chemiluminescent nitrogen detection (CLND), electrospray ionization mass spectrometry, LC-MS, accurate mass measurement, LC-MS-MS analysis, high throughput screen

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Article Details

Year: 2005
Page: [477 - 487]
Pages: 11
DOI: 10.2174/1386207054867346
Price: $65

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