In this investigation the utility of evaporative light scattering detection (ELSD) combined with HPLC-MS was demonstrated as a key component of a bioassay-guided fractionation, or “biofractionation” technique, for the evaluation of high throughput screen actives. ELSD provided on-line analyte mass information that was critical for the classification of the samples. Chemiluminescent nitrogen detection (CLND) was also evaluated for sample concentration estimation for nitrogen-containing compounds, and accurate mass LC-MS-MS analysis was employed for rapid structural confirmation and elucidation of components previously identified as active via biofractionation.
Keywords: Bioassay-guided fractionation, biofractionation evaporative light scattering detection (ELSD), evaporative light scattering detection (ELSD), chemiluminescent nitrogen detection (CLND), electrospray ionization mass spectrometry, LC-MS, accurate mass measurement, LC-MS-MS analysis, high throughput screen
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