Abstract
An inexpensive, rapid and reproducible capillary electrophoretic method has been developed and validated for the determination of metformin in pharmaceutical preparations. The method was developed utilizing a fused silica capillary (60 cm x 50 μm I.D.), phosphate buffer (50 mM, 3.0 pH)-acetonitrile (95:5, v/v) as background electrolyte (BGE), 20 kV applied voltage with UV detection at 254 nm and at a working temperature of 23±1°C. Linearity was observed in the concentration range from 100 ng/L to 5 μg/L, with a correlation coefficient (R2) of 0.9998. The limits of detection and quantification achieved were 60 and 100 ng/mL, respectively. The recovery of metformin from pharmaceutical preparations was 99.1%. These validation parameters demonstrate the precision of the method and its suitability for the determination of metformin in pharmaceutical tablet formulations.
Keywords: Metformin, capillary electrophoresis, extraction, pharmaceutical analysis, pharmaceutical tablet formulation
Combinatorial Chemistry & High Throughput Screening
Title: Analysis of Metformin Dosage Formulations by Capillary Electrophoresis at Nano Scale Detection
Volume: 10 Issue: 7
Author(s): Imran Ali, Hassan Y. Aboul-Enein and Vinod K. Gupta
Affiliation:
Keywords: Metformin, capillary electrophoresis, extraction, pharmaceutical analysis, pharmaceutical tablet formulation
Abstract: An inexpensive, rapid and reproducible capillary electrophoretic method has been developed and validated for the determination of metformin in pharmaceutical preparations. The method was developed utilizing a fused silica capillary (60 cm x 50 μm I.D.), phosphate buffer (50 mM, 3.0 pH)-acetonitrile (95:5, v/v) as background electrolyte (BGE), 20 kV applied voltage with UV detection at 254 nm and at a working temperature of 23±1°C. Linearity was observed in the concentration range from 100 ng/L to 5 μg/L, with a correlation coefficient (R2) of 0.9998. The limits of detection and quantification achieved were 60 and 100 ng/mL, respectively. The recovery of metformin from pharmaceutical preparations was 99.1%. These validation parameters demonstrate the precision of the method and its suitability for the determination of metformin in pharmaceutical tablet formulations.
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Cite this article as:
Ali Imran, Aboul-Enein Y. Hassan and Gupta K. Vinod, Analysis of Metformin Dosage Formulations by Capillary Electrophoresis at Nano Scale Detection, Combinatorial Chemistry & High Throughput Screening 2007; 10 (7) . https://dx.doi.org/10.2174/138620707782152380
DOI https://dx.doi.org/10.2174/138620707782152380 |
Print ISSN 1386-2073 |
Publisher Name Bentham Science Publisher |
Online ISSN 1875-5402 |
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