Analysis of Metformin Dosage Formulations by Capillary Electrophoresis at Nano Scale Detection

Author(s): Imran Ali, Hassan Y. Aboul-Enein, Vinod K. Gupta

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 10 , Issue 7 , 2007

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Abstract:

An inexpensive, rapid and reproducible capillary electrophoretic method has been developed and validated for the determination of metformin in pharmaceutical preparations. The method was developed utilizing a fused silica capillary (60 cm x 50 μm I.D.), phosphate buffer (50 mM, 3.0 pH)-acetonitrile (95:5, v/v) as background electrolyte (BGE), 20 kV applied voltage with UV detection at 254 nm and at a working temperature of 23±1°C. Linearity was observed in the concentration range from 100 ng/L to 5 μg/L, with a correlation coefficient (R2) of 0.9998. The limits of detection and quantification achieved were 60 and 100 ng/mL, respectively. The recovery of metformin from pharmaceutical preparations was 99.1%. These validation parameters demonstrate the precision of the method and its suitability for the determination of metformin in pharmaceutical tablet formulations.

Keywords: Metformin, capillary electrophoresis, extraction, pharmaceutical analysis, pharmaceutical tablet formulation

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Article Details

VOLUME: 10
ISSUE: 7
Year: 2007
Page: [611 - 615]
Pages: 5
DOI: 10.2174/138620707782152380
Price: $65

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