Real-Time PCR: Revolutionizing Detection and Expression Analysis of Genes

Author(s): S.A. Deepak, K.R. Kottapalli, R. Rakwal, G. Oros, K.S. Rangappa, H. Iwahashi, Y. Masuo, G.K. Agrawal

Journal Name: Current Genomics

Volume 8 , Issue 4 , 2007

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Invention of polymerase chain reaction (PCR) technology by Kary Mullis in 1984 gave birth to real-time PCR. Real-time PCR - detection and expression analysis of gene(s) in real-time - has revolutionized the 21st century biological science due to its tremendous application in quantitative genotyping, genetic variation of inter and intra organisms, early diagnosis of disease, forensic, to name a few. We comprehensively review various aspects of real-time PCR, including technological refinement and application in all scientific fields ranging from medical to environmental issues, and to plant.

Keywords: Real-time PCR, applications, disease, microorganisms, pathogen, detection, quantification, plant-microbe interaction

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Article Details

Year: 2007
Page: [234 - 251]
Pages: 18
DOI: 10.2174/138920207781386960
Price: $65

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