Directed Evolution of Oxygenases: Screening Systems, Success Stories and Challenges

Author(s): Kang Lan Tee, Ulrich Schwaneberg

Journal Name: Combinatorial Chemistry & High Throughput Screening
Accelerated Technologies for Biotechnology, Bioassays, Medicinal Chemistry and Natural Products Research

Volume 10 , Issue 3 , 2007

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Abstract:

The field of directed evolution of oxygenases (mono-, di- and epoxygenases) is rapidly advancing as an increasing number of success stories indicate. A significant number of screening systems have been developed to specifically improve oxygenase properties. Oxygenases will become very valuable biocatalysts for synthetic applications in industry when stability, cofactor and activity properties match industrial demands. This review summarizes screening systems and principles of screening systems that have been used for directed evolution of oxygenases. Sections on mutagenic conditions, mutant library size and property improvements provide a comprehensive picture on performance and limitations of current directed evolution methodologies for oxygenases. A discussion of challenges in the directed evolution of oxygenases for industrial exploitation concludes this review.

Keywords: Oxygenase, monooxygenase, dioxygenase, epoxygenase, directed evolution, high-throughput screening

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Article Details

VOLUME: 10
ISSUE: 3
Year: 2007
Page: [197 - 217]
Pages: 21
DOI: 10.2174/138620707780126723
Price: $65

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