Phosphopeptides - Chemical Synthesis, Analysis, Outlook and Limitations.

Author(s): G. K. Toth, Z. Kele, G. Varadi.

Journal Name: Current Organic Chemistry

Volume 11 , Issue 5 , 2007

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Abstract:

Protein phosphorylation is one of the most important post-translational events in cell regulation and signal transduction. Since the isolation of phosphorylated peptides from biological sources is usually not feasible, there is a need for efficient chemical phosphorylation methods to allow the study of the role of phosphorylation/dephosphorylation in biological processes. The recent developments in phosphopeptide chemistry (special protecting groups for the phosphate moiety and new amidite reagents) have provided peptide chemists with a wide variety of different strategies applicable for work with the sensitive phosphoserine, phosphothreonine and phosphotyrosine-containing peptides. This has made possible the efficient chemical preparation of longer, multiply phosphorylated, possibly cell-permeable or fluorescent residue-bearing peptides.

Keywords: Phosphorylation, tert-butyldimethylsilyl, Phosphate Protecting Groups, PSD/MALDI, collision-induced dissociation(CID)

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Article Details

VOLUME: 11
ISSUE: 5
Year: 2007
Page: [409 - 426]
Pages: 18
DOI: 10.2174/138527207780059295
Price: $58

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