Crystallization and Preliminary X-Ray Studies of the DNA-Binding Domain of Hepatocyte Nuclear Factor-6α Complexed with DNA

Author(s): Daisuke Iyaguchi, Min Yao, Nobuhisa Watanabe, Jun Nishihira, Isao Tanaka.

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 5 , 2006

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Abstract:

Hepatocyte nuclear factor 6 (HNF-6)/OC-1, a part of liver-enriched transcription factor, controls pancreas and liver development and regulates expression of several hepatic genes. DNA-binding region of HNF-6α bound to a 14-mer DNA fragment has been crystallized by the hanging drop vapor diffusion method. The crystals belong to space group P2 with unit cell parameters of a = 73.0 Å, b = 39.0 Å, c = 106.5 Å, β= 107.6 . X-ray diffraction data were collected to 2.0 Å resolution.

Keywords: HNF-6, Transcription regulator, Homeobox, ONECUT, Crystallization

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Article Details

VOLUME: 13
ISSUE: 5
Year: 2006
Page: [531 - 533]
Pages: 3
DOI: 10.2174/092986606776819592
Price: $65

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