Crystallization and Preliminary Diffraction Studies of Malate Dehydrogenase from Streptomyces aureofaciens

Author(s): Darina Mikulasova, Natasa Tomaskova, Jana Maderova, Marta Kollarova

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006


Become EABM
Become Reviewer
Call for Editor

Abstract:

Purified malate dehydrogenase (MDH) of Streptomyces aureofaciens was crystallized either in the absence or in the presence of NADH or NADPH coenzymes by hanging-drop vapour-diffusion method. An X-ray study has shown, that MDH crystals belong to space group C2221 with unit-cell parameters A = 53.2 Å, b = 104.6 Å, c = 520.0 Å, α = β = γ = 90° , MDH-NADH crystals to space group C2 with unit-cell parameters A = 51.5 Å, b = 51.5 Å, c = 256 Å, α = β = γ = 90 , and MDH-NADPH crystals to space group C2221 with unit-cell parameters A = 72, Å b = 72 Å, c = 520 Å, α = β = γ = 90° . The crystal of native MDH diffracted to 2.1 Å resolution.

Keywords: Malate dehydrogenase (MDH), Streptomyces aureofaciens, protein crystallization

Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 13
ISSUE: 2
Year: 2006
Page: [207 - 210]
Pages: 4
DOI: 10.2174/092986606775101634
Price: $65

Article Metrics

PDF: 1