Eph/Ephrin Membrane Proteins: A Mammalian Expression Vector pTIg- BOS-Fc Allowing Rapid Protein Purification

Author(s): Bryan W. Day, Fiona M. Smith, Ke Chen, Jennifer K. McCarron, Nirmitha I. Herath, Martin Lackmann, Andrew W. Boyd

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006


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Abstract:

There is an urgent need for high purity, single chain, fully functional Eph/ephrin membrane proteins. This report outlines the pTIg-BOS-Fc vector and purification approach resulting in rapid increased production of fully functional single chain extracellular proteins that were isolated with high purity and used in structure-function analysis and pre-clinical studies.

Keywords: Crystallography, protein expression, purification, pTIg-BOS-Fc

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Article Details

VOLUME: 13
ISSUE: 2
Year: 2006
Page: [193 - 196]
Pages: 4
DOI: 10.2174/092986606775101625
Price: $65

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