Structure-Based Stabilization of an Enzyme: The Case of Penicillin Acylase from Alcaligenes faecalis

Author(s): Tianwen Wang, Hu Zhu, Xingyuan Ma, Yushu Ma, Dongzhi Wei

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006

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The modeled structure of penicillin acylase from Alcaligenes faecali (AFPGA) was constructed by comparative modeling with the Modeller program. Candidate positions that could be replaced with cysteine were estimated by scanning the modeled structure of AFPGA with the program MODIP (modeling disulfide bond in protein). The mutant Q3C/P751C had A higher optimum temperature by three degrees than that of the wild type AFPGA. The half life of the double mutant Q3C/P751C at 55°C was increased by 50%. To our knowledge, this was the first structure-based genetic modification of AFPGA.

Keywords: Thermal stability, Penicillin acylase, Site-directed mutagenesis, Homology modeling, Double mutations

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Article Details

Year: 2006
Published on: 01 March, 2012
Page: [177 - 183]
Pages: 7
DOI: 10.2174/092986606775101571
Price: $65

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