Asparaginase Display of Human Cholesteryl Ester Transfer Protein (CETP) B Cell Epitopes for Inducing High Titers of Anti-CETP Antibodies In Vivo

Author(s): Qi Gaofu, Cao Rongyue, Mao Dan, Zhao Xiuyun, Wang Xuejun, Wu Jie, Liu Jingjing

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006

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The recombinant chimeric enzyme, AnsB-TTP-CETPC, comprising asparaginase, tetanus toxin helper T cell epitope and human CETP B cell epitope was expressed as A soluble protein in Escherichia coli. The purified chimeric enzyme exhibited approximate 83% activity of the native asparaginase. After immunization with three doses of chimeric enzyme, high titers of anti-CETP antibodies were induced and lasted more than eighteen weeks in mice, and could even be detected at A dilution of 1:12800 by normal ELISA assay. The specificity of anti-CETP antibody was verified by Western blot assay. After displaying on the surface of asparaginase, the weak antigenicity of CETP epitope was effectively overcome, there after A strong CETP-specific immune response was evoked in mice immunized with the chimeric enzyme. Histochemical analysis of mice kidney tissue showed that immunization with the chimeric enzyme did not cause any pathological changes in mice. Collectively, the chimeric enzyme may be further developed as A vaccine against atherosclerosis in the future.

Keywords: Asparaginase, CETP B cell epitope, tetanus toxin helper T cell epitope, atherosclerosis, vaccine

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Article Details

Year: 2006
Page: [149 - 154]
Pages: 6
DOI: 10.2174/092986606775101689
Price: $65

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