A Nonradioactive Assay for Poly(A)-Specific Ribonuclease Activity by Methylene Blue Colorimetry

Author(s): Yuan Cheng, Wei-Feng Liu, Yong-Bin Yan, Hai-Meng Zhou

Journal Name: Protein & Peptide Letters

Volume 13 , Issue 2 , 2006

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A simple nonradioactive assay, which was based on the specific shift of the absorbance maximum of methylene blue induced by its intercalation into poly(A) molecules, was developed for poly(A)-specific ribonuclease (PARN). A good linear relationship was found between the absorbance at 662 nm and the poly(A) concentration. The assay conditions, including the concentration of methylene blue, the incubation temperature and time, and the poly(A) concentration were evaluated and optimized.

Keywords: Enzyme assay, Poly(A)-specific ribonuclease, Methylene blue colorimetry, Enzyme inhibitor

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Article Details

Year: 2006
Published on: 01 March, 2012
Page: [125 - 128]
Pages: 4
DOI: 10.2174/092986606775101580
Price: $65

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