Reviews in Pharmaceutical & Biomedical Analysis

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Reviews in Pharmaceutical and Biomedical Analysis contains coverage and review of new trends and applications in all areas of pharmaceutical, biomedical and analytical chemistry. Authors have ...
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Optimizing the Generalization Ability of Artificial Neural Networks in ELISA Protocols by Employing Different Topologies and GENETIC Operators

Pp. 20-29 (10)

Constantinos Kousoulos, Yannis Dotsikas and Yannis L. Loukas


The aim of the present work was to present the ability of Artificial Neural Networks (ANN) in successfully predicting the response of an Enzyme-linked Immunosorbent assay (ELISA) from the relative input parameters and to further enhance its performance by use of different network architectures, learning algorithms and genetic operators. Representatives of three major categories of ANN topologies were investigated, namely Multilayer Feed-Forward (MLF), Generalized Feed-Forward (GenFF) and Radial Basis Function (RBF) which were trained with back-propagation with momentum and a scaled conjugate gradient learning algorithm. Tuning of the input and hidden layer size was performed by use of a genetic algorithm, while different combinations of genetic operators were used for the optimal GenFF network in order to increase its predictive ability. The major advantage of this approach was the simultaneous data-driven, modeling and optimization process which demands no a priori knowledge of variable correlations and can be employed in setting up new assays.


Division of Pharmaceutical Chemistry, Department of Pharmacy, University of Athens, Panepistimioupoli Zografou GR - 157 71, Athens, Greece.