The Role of New Technologies in Medical Microbiological Research and Diagnosis

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This e-book provides a comprehensive overview of state of the art applications of biomolecular techniques that are currently used, or are in development in the field of microbiological diagnostics ...
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Bead-Based Flow-Cytometry in Medical Microbiological Research and Diagnosis

Pp. 151-160 (10)

N. J. Verkaik, C. P. de Vogel, W. J.B. van W. and A. van Belkum

Abstract

Bead-based flow cytometry (xMAP® and xTAG® Technology, Luminex Corporation) is a recently developed technology that allows for simultaneous quantification of multiple antibodies, antigens or oligonucleotides in a single sample. The newest generation of analyzers allow multiplexing of up to 500 unique tests within a single sample, which renders this technique much less timeconsuming than conventional testing methodsologies. Numerous applications have already been developed for this technology in both medical microbiological research and diagnosis, with the number of reported applications still growing rapidly. Bead-based flow-cytometry has already firmly established itself as a supplemental and/or alternative technology to the more traditional routine diagnostic and research microbiology test platforms.

Keywords:

Flow Cytometry, Luminex, XMap, Bead-based, Median Intensity Fluorescence, Immunity.

Affiliation:

Erasmus Medical Centre Rotterdam, Department of Medical Microbiology & Infectious Diseases‘s -Gravendijkwal 230, 3015 CE Rotterdam, The Netherlands.