Abstract
Background: Worldwide, cancer is claimed a major public health problem and is another leading cause of death after ischemic heart diseases. Currently, the nutraceuticals and their biologically active phytoconstituents have received immense attention for the prevention and treatment of cancer due to origin authenticity, natural compatibility, and least toxicity.
Objective: The contemporary research venture aimed to investigate the anticancer potential of polar fractions of the Chrozophora tinctoria plant. The plant was selected due to its wide-ranging therapeutic potential having its role in wound healing, anti-inflammatory, antimicrobial, antineoplastic as well as antioxidant activities.
Method: The aerial part of the C. tinctoria was extracted with methanol and then fractionated with liquid-liquid extraction by utilizing water, butanol, ethyl acetate, chloroform, and n-hexane. In addition to total phenolic, the flavonoid contents were also determined. Phytochemical analysis was performed and maximum phenolic and flavonoid contents were obtained in chloroform and ethyl acetate fractions.
Results: The obtained fractions were assessed for potential anticancer agents by utilizing HeLa and MCF-7 cells. The most active fractions from initial anticancer screening were further investigated microscopically to determine apoptosis, production of reactive oxygen species (ROS) as well as cell cycle arrest (flow cytometry). Interestingly, the maximum cytotoxic activity in cancer cells was observed by ethyl acetate and chloroform fraction.
Conclusion: It is concluded that ethyl acetate and chloroform fractions showed the most promising anticancer activity and would be a new treatment option for the most common cancer in females.
Keywords: Chrozophora tinctorial, breast cancer, cervical cancer, flow cytometry, reactive oxygen species, flow cytometry.
Graphical Abstract
Call for Papers in Thematic Issues
Functional Food Factors and Therapeutics
The development of new rugs in disease therapy is focused on targeting molecules involved in the growth and death of cells in attracting attention to immunotherapy. In the control and treatment of disease, classical drugs, which have been used for a long time, are still widely used as primary selective ...read more
Treatment of chronic diseases with edible Natural Products. The case of cardiovascular agents"
The aim of this special issue is to highlight the importance of the use of edible natural products (both isolated pure compounds and extracts-mixtures) in the treatment of cardiovascular diseases. In this topic, it would be interesting to include all the potential researchers that have been involved in the study ...read more
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