Background: Worldwide, cancer is claimed a major public health problem and is
another leading cause of death after ischemic heart diseases. Currently, the nutraceuticals and their
biologically active phytoconstituents have received immense attention for the prevention and treatment
of cancer due to origin authenticity, natural compatibility, and least toxicity.
Objective: The contemporary research venture aimed to investigate the anticancer potential of polar
fractions of the Chrozophora tinctoria plant. The plant was selected due to its wide-ranging therapeutic
potential having its role in wound healing, anti-inflammatory, antimicrobial, antineoplastic
as well as antioxidant activities.
Method: The aerial part of the C. tinctoria was extracted with methanol and then fractionated with
liquid-liquid extraction by utilizing water, butanol, ethyl acetate, chloroform, and n-hexane. In addition
to total phenolic, the flavonoid contents were also determined. Phytochemical analysis was performed
and maximum phenolic and flavonoid contents were obtained in chloroform and ethyl acetate
Results: The obtained fractions were assessed for potential anticancer agents by utilizing HeLa and
MCF-7 cells. The most active fractions from initial anticancer screening were further investigated
microscopically to determine apoptosis, production of reactive oxygen species (ROS) as well as
cell cycle arrest (flow cytometry). Interestingly, the maximum cytotoxic activity in cancer cells
was observed by ethyl acetate and chloroform fraction.
Conclusion: It is concluded that ethyl acetate and chloroform fractions showed the most promising
anticancer activity and would be a new treatment option for the most common cancer in females.