Most of the biologically active microbial natural products and their analogs bear a
complex molecular architecture. The semisynthetic modifications and stereospecific diversity-
oriented synthesis of these native natural products to generate analogs are difficult and
time-consuming. Mutasynthesis is a powerful tool that utilizes the microorganism's genetic
and metabolic engineering skills to produce derivatives of complex natural products of microbial
origin. Mutasynthesis is based on the cellular uptake of chemically modified intermediates
from the culture media and their addition to the secondary metabolism by mutant microorganisms.
This review will describe the importance of mutasynthesis in the generation of
complex microbial secondary metabolites. We have covered a literature search on mutasynthesis
over the last ten years (2011-2020) in this review.
Keywords: Mutasynthesis, precursor directed biosynthesis, gene, biosynthesis, combinatorial, secondary metabolites.
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