Background: Anal fistula is one of the most common colorectal and perirectal diseases in
the world. Cuyuxunxi (CYXX) prescription is an efficient herbal fumigant used to promote the
surgical wound healing of anal fistulas.
Objective: This study aimed to explore the underlying molecular mechanism of CYXX prescription
on surgical wound healing of anal fistulas.
Methods: Ten patients with anal fistula were randomized into a control group or treatment group.
The wound surface of patients in the control group was rinsed by normal saline, while that in the
treatment group was rinsed by CYXX prescription. The wound tissues of patients with anal fistulas
seven days after the surgery were collected for hematoxylin-eosin (HE) staining and RNA
sequencing. The expressions of differentially expressed genes (DEGs) were validated by real-time
quantitative PCR (RT-qPCR).
Results: HE staining showed that CYXX treatment reduced the infiltration of inflammatory cells. A
total of 472 DEGs, including 141 up-regulated genes and 331 down-regulated genes, were identified.
These genes were significantly related to skin development, xenobiotic stimulus, and inflammation.
In addition, the consistency rate of RT-qPCR and sequencing results was 83.33%, which showed a
high relative reliability of the sequencing results.
Conclusion: CYXX prescription could improve epidermis repair and reduce inflammatory