In-silico Studies and Wet-Lab Validation of Camptothecin Derivatives for Anti-Cancer Activity Against Liver (HepG2) and Lung (A549) Cancer Cell Lines

Author(s): Komal Kalani, Dharmendra K. Yadav, Sarfaraz Alam, Feroz Khan, Mahendra P. Kashyap, Santosh K. Srivastava*, Aditya B. Pant

Journal Name: Current Topics in Medicinal Chemistry

Volume 21 , Issue 10 , 2021

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Graphical Abstract:



: In the present study, we have explored the utility of QSAR modelling, in silico ADMET, docking, chemical semi-synthesis, and in vitro evaluation studies for the identification of active camptothecin (CPT) derivatives against cancer-targeting human liver (HepG2) and lung (A549) cancer cell lines.

Methods: Two QSAR models were developed as screenings tools using the multiple linear regression (MLR) method followed by ADMET and docking studies. The regression coefficient (r2) and cross-validation regression coefficients (rCV2T) of the QSAR model for the HepG2 cell line was 0.95 and 0.90, respectively, and for the A549 cell line, it was 0.93 and 0.81, respectively.

Results: In silico studies show that CPT derivatives (CPT-1 and CPT-6) possess drug-like properties. Docking performed on DNA Topoisomerase-I showed significant binding affinity. Finally, predicted active derivatives were chemically semi synthesized, spectroscopically characterized, and evaluated in-vitro for cytotoxic/anticancer activity against HepG2 and A549 cell lines.

Conclusion: The experimental results are consistent with the predicted results. These findings may be of immense importance in the anticancer drug development from an inexpensive and widely available natural product, camptothecin.

Keywords: QSAR, ADME/T, Bioavailability, Docking, Camptothecin derivatives, Anticancer, Cytotoxic.

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Article Details

Year: 2021
Published on: 26 April, 2021
Page: [908 - 919]
Pages: 12
DOI: 10.2174/1568026621666210426124719
Price: $65

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