Aim: The present study was aimed to developed and optimize the self-nano emulsifying
drug delivery system of α-pinene (ALP-SNEDDS) and evaluate its in-vivo anti-Parkinson's activity.
Background: Different lipid-based drug delivery technologies have been researched to upgrade drug
bioavailability and expand their clinical adequacy upon oral administration. Self-emulsifying drug delivery
systems (SEDDS) have pulled in developing the interest specifically for self nano emulsifying
drug delivery systems (SNEDDS).
Objective: The present work was attempted to improve the bioavailability of the ALP by defining the
role of self-nano emulsifying formulations for its neuroprotective effect.
Methods: Miscibility of the ALP was estimated in various excipient components to select the optimized
combination. Self-nano emulsification, thermodynamic stability, the effect of dilution on robustness,
optical clarity, viscosity, and conductivity tests were performed. The in-vivo anti-Parkinson's
activity of the ALP-SNEDDS formulations were done using Pilocarpine antagonism induced Parkinsonism
in rodents. Behavioral tests like tremulous jaw movements, body temperature, salivation, and
lacrimation are performed.
Result: Two optimized formulations, composed of Anise oil, Tween 80, and Transcutol-HP of Oil:
Smix ratio (4:6 and 3:7) were selected. The Smix ratio for both the formulation was 2:1. The particle
size was found to consistent with the increase in dilution. The mean negative zeta potential of the formulations
was found to be increased with an increase in dilution. The TEM images of the formulations
revealed spherical shape of the droplet. The in-vitro drug release profile was found to be significant as
compared to plain ALP suspension.
Conclusion: The results of in-vivo studies indicate that nanosizing and enhanced solubilization of oral
ALP-SNEDDS formulations significantly improved the behavioral activities compared to plain ALP