Background: Despite significant biological effects, the clinical use of chrysin has been
restricted because of its poor oral bioavailability.
Objective: The purpose of the present research was to investigate the targeting potential of Mannose
decorated chrysin (5,7- dihydroxyflavone) loaded solid lipid nanocarrier (MC-SLNs) for gastric
Methods: The Chrysin loaded SLNs (C-SLNs) were developed, optimized, characterized and further
mannosylated. The C-SLNs were developed with a high shear homogenizer, optimized with 32
full factorial designs and characterized by Fourier Transform Infrared Spectroscopy (FTIR), Differential
Scanning Calorimetry (DSC), X-Ray Diffraction (XRD) and Scanning Electron Microscope
(SEM) and evaluated for particle size/polydispersity index, zeta-potential, entrapment efficiency,
% release and haemolytic toxicity. The ex-vivo cytotoxicity study was performed on gastric cancer
(ACG) and normal cell lines.
Results: DSC and XRD data predict the chrysin encapsulation in the lipid core and FTIR results
confirm the mannosylation of C-SLNs. The optimized C-SLNs exhibited a narrow size distribution
with a particle size of 285.65 nm. The % Entrapment Efficiency (%EE) and % controlled release
were found to be 74.43% and 64.83%. Once C-SLNs were coated with mannose, profound change
was observed in a dependent variable - an increase in the particle size of MC-SLNs (307.1 nm) was
observed with 62.87% release and 70.8% entrapment efficiency. Further, the in vitro studies depicted
MC- SLNs to be least hemolytic than pure chrysin and C-SLNs. MC-SLNs were most cytotoxic
and were preferably taken up ACG tumor cells as evaluated against C-SLNs.
Conclusion: These data suggested that the MC-SLNs demonstrated better biocompatibility and targeting
efficiency to treat gastric cancer.