Mannosylated Solid Lipid Nanocarriers of Chrysin to Target Gastric Cancer: Optimization and Cell Line Study

Author(s): Sonia S. Pandey*, Farhinbanu I. Shaikh, Arti R. Gupta, Rutvi J. Vaidya

Journal Name: Current Drug Delivery

Volume 18 , Issue 10 , 2021


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Abstract:

Background: Despite significant biological effects, the clinical use of chrysin has been restricted because of its poor oral bioavailability.

Objective: The purpose of the present research was to investigate the targeting potential of Mannose decorated chrysin (5,7- dihydroxyflavone) loaded solid lipid nanocarrier (MC-SLNs) for gastric cancer.

Methods: The Chrysin loaded SLNs (C-SLNs) were developed, optimized, characterized and further mannosylated. The C-SLNs were developed with a high shear homogenizer, optimized with 32 full factorial designs and characterized by Fourier Transform Infrared Spectroscopy (FTIR), Differential Scanning Calorimetry (DSC), X-Ray Diffraction (XRD) and Scanning Electron Microscope (SEM) and evaluated for particle size/polydispersity index, zeta-potential, entrapment efficiency, % release and haemolytic toxicity. The ex-vivo cytotoxicity study was performed on gastric cancer (ACG) and normal cell lines.

Results: DSC and XRD data predict the chrysin encapsulation in the lipid core and FTIR results confirm the mannosylation of C-SLNs. The optimized C-SLNs exhibited a narrow size distribution with a particle size of 285.65 nm. The % Entrapment Efficiency (%EE) and % controlled release were found to be 74.43% and 64.83%. Once C-SLNs were coated with mannose, profound change was observed in a dependent variable - an increase in the particle size of MC-SLNs (307.1 nm) was observed with 62.87% release and 70.8% entrapment efficiency. Further, the in vitro studies depicted MC- SLNs to be least hemolytic than pure chrysin and C-SLNs. MC-SLNs were most cytotoxic and were preferably taken up ACG tumor cells as evaluated against C-SLNs.

Conclusion: These data suggested that the MC-SLNs demonstrated better biocompatibility and targeting efficiency to treat gastric cancer.

Keywords: Chrysin, mannosylation, optimization, cell line study, gastric cancer, ACG.

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Article Details

VOLUME: 18
ISSUE: 10
Year: 2021
Published on: 19 March, 2021
Page: [1539 - 1549]
Pages: 11
DOI: 10.2174/1567201818666210319142206
Price: $95

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