Objective: Coronary artery disease (CAD) is one of the leading causes of morbidity and
mortality worldwide and statins are frequently prescribed in the treatment of CAD to help lower
blood cholesterol levels. Since the main enzyme involved in the metabolism of statins is CYP3A4,
we aimed to investigate the effect of CYP3A4 * 1B genotypes on plasma lipid profile in Turkish
cardiovascular disease subjects with and without obesity taking statin.
Materials and Methods: The study group consisted of 85 cardiovascular disease patients who were
prescribed statins and had routine biochemical analysis data. Polymerase chain reaction followed
by restriction fragment length polymorphism (PCR-RFLP) assay was performed for genotyping of
CYP3A4 *1B (rs2740574) polymorphism.
Results: Genotype distribution of CYP3A4 *1B polymorphism was found for homozygous wild
(AA) and homozygous polymorphic (GG) genotypes as 94.1% and 5.9%, respectively. We did not
detect patients with heterozygous genotype in our study group. We found that the mean LDL-c, TG
and TC levels were higher in patients with CYP3A4 *1B GG compared to the AA genotype. The
frequency of CYP3A4 *1B GG genotype frequency (9.5%) was detected higher in the obese patients
compared to the non-obese patients (7.7%) (χ2=0.037, p=0.85).
Conclusion: Our results demonstrate that CYP3A4 *1B homozygous polymorphic genotype distribution
tends to be higher in obese patients compared to non-obese patients with cardiovascular disease
which may point to *1B allele having a slight effect on serum lipids during statin therapy. Additional
studies with higher samples are needed for evaluating the role of CYP3A4 *1B on lipids in
patients under statin therapy.