Background: The role and mechanism of long non-coding RNA cytoskeleton regulator
(CYTOR) in Invasive Pituitary Adenomas (IPA) have not been elucidated previously.
Objective: This study aimed to investigate the interaction between CYTOR and miR-206 and their
roles in IPA using HP75 cells as the model.
Methods: The expression levels of CYTOR and miR-206 were detected by quantitative real-time
polymerase chain reaction (qRT-PCR) in IPA tissues and cell lines. The Chi-square test was used
to analyze the correlation between CYTOR expression and clinical-pathological parameters. HP75
cell proliferation was detected by Cell Counting Kit-8 assay and colony formation assay. Scratch
healing experiments and Transwell assay were used to detect migration and invasion of HP75 cells.
The relationship between CYTOR and miR-206 was predicted by bioinformatics and verified by
qRT-PCR and the dual-luciferase reporter gene method.
Results: CYTOR is up-regulated in IPA tissues and cell lines. The high expression of CYTOR is
associated with adenoma invasiveness and adenoma size of the patients. Down-regulation of CYTOR
decreases the proliferation, migration and invasion of HP75 cells, while up-regulation of
miR-206 can inhibit proliferation, migration and invasion of HP75 cells. MiR-206 is identified as a
target of CYTOR and could be negatively regulated by it in IPA.
Discussion: CYTOR, as a tumor-promoting factor, facilitates the proliferation, migration and invasion
of HP75 cells through sponging miR-206.
Conclusion: The CYTOR-miR-206 axis provides new insights into the diagnosis and treatment of