Objective: The present study aimed to explore the in-vitro anticancer potential of 5-fluorouracil
(5-FU) loaded PLGA nanogels coated with nerolidol sesquiterpene.
Methods: The emulsification-solvent evaporation technique was used for the preparation of plain
PLGA nanogels (PNGs) and 5-FU loaded PLGA nanogels (FPNGs). A surface coating of Nerolidol
(2%) sesquiterpene was employed to improve the penetration efficacy of the nanogels into the
Results: The nanogels formulation FPNGs have the size range 220±0.25% nm obtained by dynamic
light scattering. The entrapment efficiency of approx ~ 42% with a sustained-release pattern for
24 h was estimated at different pH ranges. The cell uptake and localization profile were revealed
by confocal microscopy analysis using the HaCaT cell line. MTT assay demonstrated the cell compatibility
of nanogels, confirmed by apoptosis assay depicting the apoptotic index of 0.87.
Conclusion: This study concludes that FPNGs are a promising nanogels system against skin cancer
that can be used to boost the chemo-therapeutic efficiency of bioactives with sustained and controlled
release at the desired site.