Aptamer Based Diagnosis: A Cost-Effective and Suitable Point of Care Testing Method Against SARS Coronavirus-2 (SARs-CoV-2) and Other Rapidly Spreading Diseases

Author(s): Vivek Kumar, Gulab S. Yadav, Basu D. Banerjee*

Journal Name: Current Biotechnology

Volume 10 , Issue 1 , 2021


Become EABM
Become Reviewer
Call for Editor

Graphical Abstract:


Abstract:

The current SARS coronavirus-2 (SARS-CoV-2) pandemic has raised serious concerns regarding the inefficiency of available diagnostic methods for rapid and efficient detection of the disease. It is agreed widely that Real-time Polymerase Chain Reaction (RT-PCR) and antibody- based assays have several limitations that did not help much in preventing the exponential spread of the disease in a short span of period. Unarguably, the world needs “new-generation diagnostic intervention(s)” against rapidly spreading disease like SARS-CoV-2. We have presented an aptamer-based strategy as a possible point of care testing for the diagnosis of the disease. It has several advantages over current tools available and can be used for efficient combating by the mean of quick,cost-effective and much more accurate diagnostic against the enigmatic SARS-CoV-2 disease and similar pandemic which world may possibly encounter in the future.

Keywords: SARS-CoV-2, RT-PCR, diagnosis, point of care testing, aptamer, ELONA.

[1]
Loeffelholz MJ, Tang YW. Laboratory diagnosis of emerging human coronavirus infections - the state of the art. Emerg Microbes Infect 2020; 9(1): 747-56.
[http://dx.doi.org/10.1080/22221751.2020.1745095] [PMID: 32196430]
[2]
Li Z, Yi Y, Luo X, et al. Development and clinical application of a rapid IgM-IgG combined antibody test for SARS-CoV-2 infection diagnosis. J Med Virol 2020; 92(9): 1518-24.
[http://dx.doi.org/10.1002/jmv.25727] [PMID: 32104917]
[3]
Xiao AT, Tong YX, Zhang S. False negative of RT-PCR and prolonged nucleic acid conversion in COVID-19: Rather than recurrence. J Med Virol 2020; 92(10): 1755-6.
[http://dx.doi.org/10.1002/jmv.25855] [PMID: 32270882]
[4]
Zu ZY, Jiang MD, Xu PP, et al. Coronavirus Disease 2019 (COVID-19): A Perspective from China. Radiology 2020; 296(2): E15-25.
[http://dx.doi.org/10.1148/radiol.2020200490] [PMID: 32083985]
[5]
Udugama B, Kadhiresan P, Kozlowski HN, et al. Diagnosing COVID-19: The Disease and Tools for Detection. ACS Nano 2020; 14(4): 3822-35.
[http://dx.doi.org/10.1021/acsnano.0c02624] [PMID: 32223179]
[6]
Dhiman A, Kalra P, Bansal V, Bruno JG, Sharma TK. Aptamer-based point-of-care diagnostic platforms. Sens Actuators B Chem 2017; 246: 535-53.
[http://dx.doi.org/10.1016/j.snb.2017.02.060]
[7]
Zou X, Wu J, Gu J, Shen L, Mao L. Application of Aptamers in Virus Detection and Antiviral Therapy. Front Microbiol 2019; 10: 1462.
[http://dx.doi.org/10.3389/fmicb.2019.01462] [PMID: 31333603]
[8]
Yadav GS, Kumar V, Aggarwal NK. Aptamers: Biotechnological applications of a next generation tool. 1st ed. Springer Nature Singapore Pte Ltd. 2019.
[http://dx.doi.org/10.1007/978-981-13-8836-1]
[9]
Wu S, Liu L, Duan N, Li Q, Zhou Y, Wang Z. Aptamer-Based Lateral Flow Test Strip for Rapid Detection of Zearalenone in Corn Samples. J Agric Food Chem 2018; 66(8): 1949-54.
[http://dx.doi.org/10.1021/acs.jafc.7b05326] [PMID: 29425043]
[10]
Dalirirad S, Steckl AJ. Aptamer-based lateral flow assay for point of care cortisol detection in sweat. Sens Actuators B Chem 2019; 282: 79-86.
[http://dx.doi.org/10.1016/j.snb.2018.11.161]
[11]
Dalirirad S, Steckl AJ. Lateral flow assay using aptamer-based sensing for on-site detection of dopamine in urine. Anal Biochem 2020; 596: 113637.
[http://dx.doi.org/10.1016/j.ab.2020.113637] [PMID: 32087129]
[12]
Huang CC, Huang YF, Cao Z, Tan W, Chang HT. Aptamer-modified gold nanoparticles for colorimetric determination of platelet-derived growth factors and their receptors. Anal Chem 2005; 77(17): 5735-41.
[http://dx.doi.org/10.1021/ac050957q] [PMID: 16131089]
[13]
Guthrie JW, Hamula CLA, Zhang H, Le XC. Assays for cytokines using aptamers. Methods 2006; 38(4): 324-30.
[http://dx.doi.org/10.1016/j.ymeth.2006.01.001] [PMID: 16495077]
[14]
Ramos E, Piñeiro D, Soto M, et al. A DNA aptamer population specifically detects Leishmania infantum H2A antigen. Lab Invest 2007; 87(5): 409-16.
[http://dx.doi.org/10.1038/labinvest.3700535] [PMID: 17334412]
[15]
Reverdatto S, Burz DS, Shekhtman A. Peptide aptamers: development and applications. Curr Top Med Chem 2015; 15(12): 1082-101.
[http://dx.doi.org/10.2174/1568026615666150413153143] [PMID: 25866267]
[16]
Wang J, Munir A, Li Z, Zhou HS. Aptamer-Au NPs conjugates-accumulated methylene blue for the sensitive electrochemical immunoassay of protein. Talanta 2010; 81(1-2): 63-7.
[http://dx.doi.org/10.1016/j.talanta.2009.11.035] [PMID: 20188888]
[17]
Kuang H, Chen W, Xu D, et al. Fabricated aptamer-based electrochemical “signal-off” sensor of ochratoxin A. Biosens Bioelectron 2010; 26(2): 710-6.
[http://dx.doi.org/10.1016/j.bios.2010.06.058] [PMID: 20643539]
[18]
Zheng J, Feng W, Lin L, et al. A new amplification strategy for ultrasensitive electrochemical aptasensor with network-like thiocyanuric acid/gold nanoparticles. Biosens Bioelectron 2007; 23(3): 341-7.
[http://dx.doi.org/10.1016/j.bios.2007.04.015] [PMID: 17583489]
[19]
Baker BR, Lai RY, Wood MS, Doctor EH, Heeger AJ, Plaxco KW. An electronic, aptamer-based small-molecule sensor for the rapid, label-free detection of cocaine in adulterated samples and biological fluids. J Am Chem Soc 2006; 128(10): 3138-9.
[http://dx.doi.org/10.1021/ja056957p] [PMID: 16522082]
[20]
Cheng AKH, Ge B, Yu HZ. Aptamer-based biosensors for label-free voltammetric detection of lysozyme. Anal Chem 2007; 79(14): 5158-64.
[http://dx.doi.org/10.1021/ac062214q] [PMID: 17566977]
[21]
Hogan CA, Sahoo MK, Huang C, et al. Five-minute point-of-care testing for SARS-CoV-2: Not there yet. J Clin Virol 2020; 128: 104410.
[http://dx.doi.org/10.1016/j.jcv.2020.104410] [PMID: 32403009]
[22]
Wongphatcharachai M, Wang P, Enomoto S, et al. Neutralizing DNA aptamers against swine influenza H3N2 viruses. J Clin Microbiol 2013; 51(1): 46-54.
[http://dx.doi.org/10.1128/JCM.02118-12] [PMID: 23077124]
[23]
Alshammari TM, Al-Hassan AA, Hadda TB, Aljofan M. Comparison of different serum sample extraction methods and their suitability for mass spectrometry analysis. Saudi Pharm J 2015; 23(6): 689-97.
[http://dx.doi.org/10.1016/j.jsps.2015.01.023] [PMID: 26702265]


Rights & PermissionsPrintExport Cite as

Article Details

VOLUME: 10
ISSUE: 1
Year: 2021
Published on: 23 February, 2021
Page: [3 - 6]
Pages: 4
DOI: 10.2174/2211550110666210224095929
Price: $65

Article Metrics

PDF: 302
HTML: 2