Objective: The aim of present study was to formulate chitosan microspheres loaded with
ethanolic extract of Lens culinaris Medikus (L.culinaris) seeds (ME) and to explore its anticancer
potential against lung cancer (A549) cell line.
Methods: Central composite design was applied to prepare and optimise the chitosan microspheres.
The prepared microspheres were evaluated for its physicochemical characterisation, in
vitro drug release and anti-cancer potential in vitro.
Results: L.culinaris loaded chitosan microspheres were prepared successfully with suitable particle
size, entrapment efficiency and drug release. The developed ME were spherical shaped with the
particle size of 2.08 μm. The drug entrapment efficiency and cumulative drug release was found
1.58±0.02% and 81.95±0.35%, respectively. Differential scanning calorimetry studies revealed no
interaction between drugs and polymers used. The cytotoxic effect of the optimised formulation revealed
a significant response as compared to the ethanolic extract of L.culinaris seeds (IC50: 22.56
μg/ml vs. 63.58 μg/ml), which was comparable to that of reference drug, doxorubicin (22 μg/ml).
These observations demonstrate that the optimised microspheres are effective against lung cancer
Conclusion: The significant cytotoxic response of the developed microspheres may be attributed
due to its low particle size, high entrapment efficiency and prolonged drug release profile.