Aims: This study was carried out to study the optimized condition for microalgae cultivation
in terms of light intensity, and nutrient supply. Also, use of a carbon source was studied to
optimize the microalgae growth to produce microalgae with a high biomass productivity and a high
Background: Algae can be categorized into macroalgae and microalgae. Commonly, microalgae
are used to produce biodiesel since microalgae can yield 5000-15000 of oil gallons compared to
plant-based biomass as feedstock produced 50-500 oil gallon. Furthermore, microalgae do not face
any food crisis and can be cultivated in any wasteland that is not suitable for agriculture throughout
the year, compared to crops. Microalgae can also be cultivated in freshwater, saline water and
Methods: Microalgae cultivation was carried out with microalgae culture labelled as MX1, MX2,
MX3, MX4 and were cultivated under high light intensities, whereas MY1, MY2, MY3, MY4
were cultivated under medium light intensity and MZ1, MZ2, MZ3 MZ4 became control culture
that was cultivated under high light intensities and no light condition.
Results: The effect of light intensity, NPK fertilizer, and glucose on microalgae’s biomass production
will be observed simultaneously. At the end of cultivation, MX2 obtained the highest biomass
of 97.186 g. The oil extraction yield is 9.66%. GC-MS analysis showed the presence of UFA and
PUFA in the oil.
Conclusion: Thus, future research is needed to improve the technique to increase the microalgae
biomass and lipid to become the potential feedstock for the production of biodiesel.