Curcumin Efficacy in a Serum/glucose Deprivation-induced Neuronal PC12 Injury Model

(E-pub Ahead of Print)

Author(s): Tahereh Farkhondeh, Milad Ashrafizadeh, Mohsen Azimi-Nezhad, Fariborz Samini, Michael Aschner, Saeed Samarghandian*

Journal Name: Current Molecular Pharmacology


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Abstract:

Background: Glucose/serum deprivation (GSD), has been used for understanding molecular mechanisms of neuronal damage during ischemia. It has been suggested that curcumin may improve neurodegenerative diseases.

Aim: In this study, the protective effects of curcumin and its underlying mechanisms were investigated in PC12 cells upon GSD-induced stress.

PC12 cells were cultured in DMEM overnight and then incubated in GSD condition for either 6 or 12h. GSD-treated cells were pretreated with various concentrations of curcumin (10, 20, and 40 μM) for 5h. The cell viability, apoptosis, reactive oxygen species (ROS) level, oxidative stress, expression of apoptosis-related genes, and IL-6 were determined.

Results: Curcumin increased cell viability and caused an anti-apoptotic effect in PC12 cells exposed for 12h to GSD . Curcumin also increased antioxidant enzyme expression, suppressed lipid peroxidation, and decreased interleukin-6 secretion in PC12 cells subjected to GSD. In addition, pretreatment with curcumin down-regulated pro-apoptotic (Bax), and up-regulated antiapoptotic (Bcl2) mediators.

Conclusion: Curcumin mitigates many of the adverse effects of ischemia, and therefore, should be considered as an adjunct therapy in ischemic patients.

Keywords: apoptosis; curcumin; cytotoxicity; inflammation; oxidative stress; PC12 cell; serum/glucose deprivation.

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Article Details

Published on: 03 February, 2021
(E-pub Ahead of Print)
DOI: 10.2174/1874467214666210203211312
Price: $95

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