Background: The bis(indolyl)methanes (BIMs) scaffold is reported for wide varieties of pharmacological
profiles, including antibacterial, anti-proliferative, anticancer, cytotoxic, insecticidal, analgesic,
antioxidant, and anti-inflammatory agents.
Materials and Methods: A series of bis(indolyl)methanes have been synthesized by a greener and newer
approach using the reaction of different substituted aldehydes and indole in the presence of an easily
available and biodegradable base such as piperidine in acetic acid at room temperature and characterized
with ultraviolet–visible spectrophotometry (UV–Vis or UV/Vis), Fourier-transform infrared spectroscopy
(FTIR), nuclear magnetic resonance spectroscopy (NMR), etc. The antibacterial and antifungal activities
were also carried out against Staphylococcus aureus (RCMB 000106) and Bacillis subtilis (RCMB
000107), as two Gram-positive bacterial strains, and Salmonella typhi and Escherichia coli (RCMB
000103), as two Gram-negative bacterial strains. Fungal species such as Candida Albicans, Penicillium
chrysogenum, Aspergillus niger were also used for in vitro antifungal evaluation. All our newly synthesized
14 compounds (4a-4n) were subjected for anti-inflammatory activity in vitro and compared with the
known standard drug aceclofenac.
Results: Our newly synthesized compounds showed good to moderate antibacterial agents, in silico ADMET,
and anti-inflammatory profiles.
Conclusion: We hope that our current study would aid future developments of bis(indolyl)methanes as
antibacterial and anti-inflammatory agents.