Background: Neurodegenerative diseases have become an increasing cause of various
disabilities worldwide, followed by aging, including Parkinson’s disease (PD). Parkinson’s disease
is a degenerative brain disorder distinguished by growing motor & non-motor failure due to the degeneration
of medium-sized spiked neurons in the striatum region. Rotenone is often employed to
originate the animal model of PD. It is a powerful blocker of mitochondrial complex-I, mitochondrial
electron transport chain that reliably produces Parkinsonism-like symptoms in rats. Rice bran
(RB) is very rich in polyunsaturated fatty acids (PUFA) and nutritionally beneficial compounds,
such as γ-oryzanol, tocopherols, and tocotrienols and sterols are believed to have favorable outcomes
on oxidative stress & mitochondrial function.
Objective: The present study has been designed to explore RB extract’s effect against rotenone-induced
neurotoxicity in rats.
Methods: In the present study, Rotenone (2 mg/kg, s.c) was administered systemically for 28 days.
The hexane extract of RB was prepared using Soxhlation. Hexane extract (250 & 500 mg/kg) was
administered per oral for 28 days in rotenone-treated groups. Behavioral parameters (grip strength,
motor coordination, locomotion, and catalepsy) were conducted on the 7th, 14th, 21st, and 28th day.
Animals were sacrificed on the 29th day for biochemical estimation in the striatum and cortex.
Results: This study demonstrates significant alteration in behavioral parameters, oxidative burden
(increased lipid peroxidation, nitrite concentration, and decreased glutathione, catalase, SOD) in
rotenone-treated animals. Administration of hexane extract of RB prevented the behavioral, biochemical
alterations induced by rotenone. The current research has been sketched to inspect RB extract’s
effect against rotenone-developed neurotoxicity in rats.
Conclusion: The findings support that PD is associated with impairments in motor activity. The results
also suggest that the nutraceutical rice bran that contains γ-oryzanol, Vitamin-E, ferulic acid
etc., may underlie the adjuvant susceptibility towards rotenone-induced PD in experimental rats.