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ISSN (Print): 1573-4129
ISSN (Online): 1875-676X

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General Research Article

Comprehensive Study on the Chemical Profile and Anti-Tumor Activity of Secondary Metabolites Produced by Aspergillus niger

Author(s): Yung-Husan Chen*, Pinghong Chen, Ching-Hsu Yang, Xiuna Wu, Lianzhong Luo and Weiyun Zhang*

Volume 18, Issue 1, 2022

Published on: 21 January, 2021

Page: [141 - 160] Pages: 20

DOI: 10.2174/1573412917666210121152317

Price: $65

Abstract

Objective: Chemical investigation of the extract of sponge-derived fungus, Aspergillus niger, was performed by liquid chromatography coupled with QExactive mass spectrometry for the first time.

Method: A total number of 444 constituents were detected, 288 of which were identified positively or tentatively by the comprehensive utilization of accurate molecular weight and fragmentation information acquired from quadrupole orbitrap mass spectrometry. The identified compounds were divided into several types, namely, organic acid, alkaloid, saccharide, amino acid and cyclopeptide, terpenoid, polyketone, phenylpropanoid and other types of compounds. Systematic diagnostic ions and featured fragment patterns were summarized for each type, based on which 8 novel compounds belonging to the same type were characterized.

Results: This work provided a rapid approach for the research of micro constituents in a complex analyte. Furthermore, the anti-tumor activity of the extract was evaluated on two different cell lines-Bel-7402 and Hela-S3 in vitro. The tumor-inhibitory effect of the Aspergillus niger extract was confirmed and may be mainly derived from its pro-apoptotic action. Moreover, the extract exerted more significant cytotoxicity in Bel-7402 cells than Hela-S3 cells, indicative of its selectivity on specific tumor cells.

Conclusion: The evidence suggested that the Aspergillus niger extract may potentially serve as a remedy for the prevention and therapy of hepatic and breast carcinoma.

Keywords: Aspergillus niger, LC-MS, anti-tumor, marine fungi, metabolites, liquid chromatography.

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[1]
Debbab, A.; Aly, A.H.; Proksch, P. Fungal Divers., 2011, 49, 1-12.
[http://dx.doi.org/10.1007/s13225-011-0114-0]
[2]
Rateb, M.E.; Ebel, R. Secondary metabolites of fungi from marine habitats. Nat. Prod. Rep., 2011, 28(2), 290-344.
[http://dx.doi.org/10.1039/c0np00061b] [PMID: 21229157]
[3]
Debbab, A.; Aly, A.H.; Lin, W.H.; Proksch, P. J. Microbiol. Biotechnol., 2010, 3, 544-563.
[http://dx.doi.org/10.1111/j.1751-7915.2010.00179.x]
[4]
Saleem, M.; Ali, M.S.; Hussain, S.; Jabbar, A.; Ashraf, M.; Lee, Y.S. Marine natural products of fungal origin. Nat. Prod. Rep., 2007, 24(5), 1142-1152.
[http://dx.doi.org/10.1039/b607254m] [PMID: 17898901]
[5]
Fenical, W. Drugs from the Sea; Fusetani, N., Ed.; Karger: Basel, 2000, pp. 6-29.
[6]
Faulkner, D. J. Nat. Prod. Rep., 2002, 19, 1-48.
[7]
Proksch, P.; Edrada, R.A.; Ebel, R. Drugs from the seas - current status and microbiological implications. Appl. Microbiol. Biotechnol., 2002, 59(2-3), 125-134.
[http://dx.doi.org/10.1007/s00253-002-1006-8] [PMID: 12111137]
[8]
Proksch, P.; Ebel, R.; Edrada, R.A.; Wray, V.; Steube, K. Sponges; Muller, W.E.G., Ed.; Springer: Berlin, 2003, pp. 117-142.
[9]
Cuomo, V.; Palomba, I.; Perretti, A.; Guerriero, A.; D’Ambrosio, M.; Pietra, F. J. Mar. Biotechnol., 1995, 2, 199-204.
[10]
Hiort, J.; Maksimenka, K.; Reichert, M.; Perović-Ottstadt, S.; Lin, W.H.; Wray, V.; Steube, K.; Schaumann, K.; Weber, H.; Proksch, P.; Ebel, R.; Müller, W.E.; Bringmann, G. New natural products from the sponge-derived fungus Aspergillus niger. J. Nat. Prod., 2004, 67(9), 1532-1543.
[http://dx.doi.org/10.1021/np030551d] [PMID: 15387655]
[11]
Varoglu, M.; Crews, P. Biosynthetically diverse compounds from a saltwater culture of sponge-derived Aspergillus niger. J. Nat. Prod., 2000, 63(1), 41-43.
[http://dx.doi.org/10.1021/np9902892] [PMID: 10650076]
[12]
Abrell, L.M.; Borgeson, B.; Crews, P. Tetrahedron Lett., 1996, 37, 2331-2334.
[http://dx.doi.org/10.1016/0040-4039(96)00277-8]
[13]
Bugni, T.S.; Abbanat, D.; Bernan, V.S.; Maiese, W.M.; Greenstein, M.; Van Wagoner, R.M.; Ireland, C.M. Yanuthones: novel metabolites from a marine isolate of Aspergillus niger. J. Org. Chem., 2000, 65(21), 7195-7200.
[http://dx.doi.org/10.1021/jo0006831] [PMID: 11031048]
[14]
Belofsky, G.N.; Jensen, P.R.; Renner, M.K.; Fenical, W. Tetrahedron, 1998, 54, 1715-1724.
[http://dx.doi.org/10.1016/S0040-4020(97)10396-9]
[15]
Toske, S.G.; Jensen, P.R.; Kauffman, C.A.; Fenical, W. Tetrahedron, 1998, 54, 13459-13466.
[http://dx.doi.org/10.1016/S0040-4020(98)00829-1]
[16]
Rahbaek, L.; Breinholt, J.; Circumdatins, D. E, and F: further fungal benzodiazepine analogues from aspergillus ochraceus. J. Nat. Prod., 1999, 62(6), 904-905.
[http://dx.doi.org/10.1021/np980495u] [PMID: 10395516]
[17]
Li, Y.; Li, X.; Kim, S.K.; Kang, J.S.; Choi, H.D.; Rho, J.R.; Son, B.W. Golmaenone, a new diketopiperazine alkaloid from the marine-derived fungus Aspergillus sp. Chem. Pharm. Bull. (Tokyo), 2004, 52(3), 375-376.
[http://dx.doi.org/10.1248/cpb.52.375] [PMID: 14993767]
[18]
Whyte, A.C.; Gloer, J.B.; Wicklow, D.T.; Dowdw, P.F. Sclerotiamide: a new member of the paraherquamide class with potent antiinsectan activity from the sclerotia of Aspergillus sclerotiorum. J. Nat. Prod., 1996, 59(11), 1093-1095.
[http://dx.doi.org/10.1021/np960607m] [PMID: 8946752]
[19]
Qian-Cutrone, J.; Huang, S.; Shu, Y.Z.; Vyas, D.; Fairchild, C.; Menendez, A.; Krampitz, K.; Dalterio, R.; Klohr, S.E.; Gao, Q. Stephacidin A and B: two structurally novel, selective inhibitors of the testosterone-dependent prostate LNCaP cells. J. Am. Chem. Soc., 2002, 124(49), 14556-14557.
[http://dx.doi.org/10.1021/ja028538n] [PMID: 12465964]
[20]
Rungprorn, W.; Siwu, E.R.O.; Lambert, L.K.; Dechsakulwatana, C.; Barden, M.C.; Kokpol, U.; Blanchfield, J.T.; Kita, M.; Garson, M.J. Tetrahedron, 2008, 64, 3147.
[http://dx.doi.org/10.1016/j.tet.2008.01.089]
[21]
Tian, YQ; Lin, XP; Wang, Z Asteltoxins with antiviral activities from the marine sponge-Derived fungus aspergillus sp., 2015, 21(1)E34.
[http://dx.doi.org/10.3390/molecules21010034]
[22]
Chen, M.; Shao, C.L.; Kong, C.J.; She, Z.G.; Wang, C.Y. A new anthraquinone derivative from a goronian-derived fungus Aspergillus sp. Chem. Nat. Compd., 2014, 50, 617-620.
[http://dx.doi.org/10.1007/s10600-014-1037-2]
[23]
Chen, M.; Fu, X.M.; Kong, C.J.; Wang, C.Y. Nucleoside derivatives from the marine-derived fungus Aspergillus versicolor. Nat. Prod. Res., 2014, 28(12), 895-900.
[http://dx.doi.org/10.1080/14786419.2014.891114] [PMID: 24670197]

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