Background: With the increasing prevalence of diabetes in recent years, diabetic nephropathy (DN) has also
become a dangerous disease that greatly endangers human health. The study was designed to determine the mechanism and
effect of Electro-acupuncture (EA) in alleviating DN-induced inflammation.
Methods: Mice were intraperitoneally injected with STZ (streptozotocin, 50 mg/kg, S0130, Sigma, St. Louis, MO, USA) for
five consecutive days. After 12 weeks after induction, blood glucose levels were measured (>300 mg/dL).
Results: The serum levels of IL-1β and IL-6 were decreased in EA-treated DN mice, and EA protected renal injury. EA
could suppress HMGB1/NLRP3/NF-κB pathway. HMGB1 inhibition increased the anti-inflammatory effects of EA on DN
by suppressing NLRP3/NF-κB pathway. The activation of HMGB1 attenuated the anti-inflammatory effects of EA on DN
by inducing NLRP3/NF-κB pathway.
Conclusions: These results suggested that EA protected DN-induced inflammation through suppression of NLRP3