Aim and Objective: We synthesized eicosapentaenoic acid-enriched phosphatidylcholine
(EPA-PC) and investigated its effect on the production of lipopolysaccharide (LPS)-
induced cytokines in murine splenocytes.
Material and Methods: The culture supernatants of splenocytes, which was exposed to EPA-PC
along with LPS, was harvested to determine the production of cytokines [interleukin (IL)-4 , IL-5,
IL-6, interferon (IFN)-γ, IL-2 and IL-12/IL-23(p40)]. Cytokines were measured using enzymelinked
immunosorbent assay (ELISA).
Results: The co-administration of EPA-PC with LPS resulted in a significantly lower IFN-γ
expression than that observed with LPS alone (p < 0.01). Moreover, treatment with EPA-PC and
LPS significantly decreased IL-2, IL-6 and IL-12/IL-23(p40) expression (p < 0.01). Coadministration
of EPA-PC at a concentration of 0.3 μg/mL with LPS resulted in a higher IL-5
expression after 24 hr of treatment when compared to LPS alone (p < 0.05).
Conclusion: These results suggest that EPA-PC is more effective in decreasing the expression of
pro-inflammatory cytokines [IL-2, IFN-γ, IL-6 and IL-12/IL-23(p40)] upon induction of